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KMID : 0352719990230010050
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Journal of Ginseng Research
1999 Volume.23 No. 1 p.50 ~ p.54
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Purification and Characterization of Ginsenoside-β-Glucosidase
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1Hongshan Yu
1Xiaoqun Ma/1Fenxie Jin/2Youn Guo
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Abstract
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In this paper, the saponin enzymatic hydrolysis of ginsenoside Rg3 was studied. The ginsenoside?β?glucosidase from FFCDL-48 strain mainly hydrolyzed the ginsenoside Rg3 to Rh2, the enzyme from FFCDL-00 strain hydrolyzed Rg3 to the mixture of Rh2 and protopanaxadiol (aglycon). The ginsenoside?β?glucosidase from FFCDL-48 strain was purified with a column of DEAE-Cellulose to one spot in the SDS polyacrylamide gel electrophoresis. During the purification, the enzyme specific acitvity was increased about 10 times. The purified ginsenoside?β?glucosidase can hydrolyze the Rg3 to Rh2, but do not hydrolyze the p?nitrophenyl?β?glucoside which is a substrate of original exocellulase such as β?glucosidase of cellulose. The molecular weight of ginsenoside?β?glucosidase was 34,000, the optimal temperature of enzyme reaction was 50?C, and the optimal pH was 5.0.
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KEYWORD
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Ginsenoside Rg3, Rh2, ginsenoside ??-glucosidase, ginsenoside hydrolysis,
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